The activity of the tricarboxylate (citrate) carrier has been assayed in intact liver mitochondria from yellow eel (Anguilla anguilla) and compared to that from rat. The eel-citrate carrier specific activity was approximately 1.7-fold higher than that assayed in rat-liver mitochondria. The content of the main mitochondrial phospholipids, phosphatidylethanolamine and phosphatidylcholine, did not show a significant difference between the two species, while in eel a higher cardiolipin level was observed. Fatty acid composition of eel-liver mitochondrial phospholipids was characterised by a large amount of unsaturated fatty acids, dominated by octadecaenoic acid (C18:1n-9) and docosahexaenoic acid (C22:6n-3). The cardiolipin fatty acid pattern of eel-liver mitochondria showed, with respect to the rat, a higher C20:5n-3 and C22:6n-3 content and a lower amount of C18:2n-6 and C20:4n-6. A noticeable activity of lipogenic enzymes was also detected in eel liver cytosol. The results of this study suggest that the remarkable activity of the citrate carrier in eel-liver mitochondria can most likely be ascribed to a considerable cardiolipin level. A covariance of citrate carrier and lipogenic enzyme activities was observed. © 2002 Elsevier Science Inc. All rights reserved.
Citrate carrier activity and cardiolipin level in eel (Anguilla anguilla) liver mitochondria
Giudetti A. M.;Siculella L.;Gnoni G. V.
2002-01-01
Abstract
The activity of the tricarboxylate (citrate) carrier has been assayed in intact liver mitochondria from yellow eel (Anguilla anguilla) and compared to that from rat. The eel-citrate carrier specific activity was approximately 1.7-fold higher than that assayed in rat-liver mitochondria. The content of the main mitochondrial phospholipids, phosphatidylethanolamine and phosphatidylcholine, did not show a significant difference between the two species, while in eel a higher cardiolipin level was observed. Fatty acid composition of eel-liver mitochondrial phospholipids was characterised by a large amount of unsaturated fatty acids, dominated by octadecaenoic acid (C18:1n-9) and docosahexaenoic acid (C22:6n-3). The cardiolipin fatty acid pattern of eel-liver mitochondria showed, with respect to the rat, a higher C20:5n-3 and C22:6n-3 content and a lower amount of C18:2n-6 and C20:4n-6. A noticeable activity of lipogenic enzymes was also detected in eel liver cytosol. The results of this study suggest that the remarkable activity of the citrate carrier in eel-liver mitochondria can most likely be ascribed to a considerable cardiolipin level. A covariance of citrate carrier and lipogenic enzyme activities was observed. © 2002 Elsevier Science Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
