Disposable Molecularly Imprinted Polymer-Modified Screen-Printed Electrodes for Rapid Electrochemical Detection of l-Kynurenine in Human Urine

L-Kynurenine (L-Kyn) is an endogenous metabolite produced in the catabolic route of L-Tryptophan (L-Trp), and it is a potential biomarker of several immunological disorders. Thus, the development of a fast and cheap technology for the specific detection of L-Kyn in biological f luids is of great relevance, especially considering its recent correlation with SARS-CoV-2 disease progression. Herein, a disposable screen-printed electrode based on a molecularly imprinted polymer (MIP) has beenconstructed: the o-Phenylenediamine monomer, in the presence of L-Kyn as a template with a molar ratio of monomer/template of 1/4, has been electropolymerized on the surface of a screen-printed carbon electrode (SPCE). The optimized kyn-MIP-SPCE has been characterized via cyclic voltammetry (CV), using [Fe(CN)6)]3−/4− as a redox probe and a scanning electron microscopy (SEM) technique. After the optimization of various experimental parameters, such as the number of CVelectropolymerization cycles, urine pretreatment, electrochemical measurement method and incubation period, L-Kyn has been detected in standard solutions via square wave voltammetry (SWV) with a linear range between 10 and 100 µM (R2 = 0.9924). The MIP-SPCE device allowed L-Kyn detection in human urine in a linear range of 10–1000 µM (R2 = 0.9902) with LOD and LOQ values of 1.5 and 5 µM, respectively. Finally, a high selectivity factor α (5.1) was calculated for L-Kyn toward L-Trp. Moreover, the Imprinting Factor obtained for L-Kyn was about seventeen times higher than the IF calculated for L-Trp. The developed disposable sensing system demonstrated its potential application in the biomedical field.