Natural anti-microbial compounds are a valid and safe alternative to agrochemicals to control plants pathologies and post-harvest fruit diseases. Safety and efficacy of these new biopesticides could be enhanced through micro/nanoencapsulation methods and nanomaterials exploitation. In this study, in vitro and in vivo tests were performed to evaluate the effects of pomegranate peel extract-loaded nanoparticles on Botrytis cinerea. CaCO3 nanocrystals were selected as carriers and the pomegranate peel extract loading was obtained through physical adsorption. Fruit and vegetable products were obtained from a commercial market, while B. cinerea was a strain of CRA-DC Roma collection. In vitro tests were achieved by measuring radial growth on Petri dishes. In vivo tests were performed immersing fruit and vegetable products for 30 s in a volume of the respective solutions. Disease severity (DS), disease incidence (DI) and FT-NIR spectra were recorded. CaCO3 nanocrystals effectively absorb extract producing pomegranate (Punica granatum) peel extract-loaded nanoparticles (PCN). In vitro tests showed the ability of PCN to control B. cinerea. The effectiveness was also established by the treatment on plant products to extend the shelf life. Our study suggests that PCN could be an alternative to synthetic products.
Effect of Nano Particles of Pomegranate Peel Extract on Shelf Life of Some Fruit and Vegetable Products
Baldassarre, FrancescaSecondo
;Ciccarella, GiuseppeUltimo
Funding Acquisition
2022-01-01
Abstract
Natural anti-microbial compounds are a valid and safe alternative to agrochemicals to control plants pathologies and post-harvest fruit diseases. Safety and efficacy of these new biopesticides could be enhanced through micro/nanoencapsulation methods and nanomaterials exploitation. In this study, in vitro and in vivo tests were performed to evaluate the effects of pomegranate peel extract-loaded nanoparticles on Botrytis cinerea. CaCO3 nanocrystals were selected as carriers and the pomegranate peel extract loading was obtained through physical adsorption. Fruit and vegetable products were obtained from a commercial market, while B. cinerea was a strain of CRA-DC Roma collection. In vitro tests were achieved by measuring radial growth on Petri dishes. In vivo tests were performed immersing fruit and vegetable products for 30 s in a volume of the respective solutions. Disease severity (DS), disease incidence (DI) and FT-NIR spectra were recorded. CaCO3 nanocrystals effectively absorb extract producing pomegranate (Punica granatum) peel extract-loaded nanoparticles (PCN). In vitro tests showed the ability of PCN to control B. cinerea. The effectiveness was also established by the treatment on plant products to extend the shelf life. Our study suggests that PCN could be an alternative to synthetic products.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
