The aim of the present work was to study the sensitivity of the enzyme carbonic anhydrase (CA) to chemical pollution in Mytilus galloprovincialis digestive gland in the context of a multimarker approach in view of ecotoxicological biomonitoring and assessment application. The study was carried out by an active biomonitoring, based on the use of caged organisms from a single population, translocated to the areas of interest, the Augusta-Melilli-Priolo industrial site (eastern Sicily, Italy) and Brucoli (eastern Sicily, Italy) not affected by industrial contamination and selected as a reference site. Mussels exposed in field in Augusta showed a significant increase in the digestive gland CA activity and expression compared to the animals caged in the control site of Brucoli. The CA response in animals from the polluted site was paralleled by the increase of the fluorescence of LysoSensor green charged cells, indicative of proliferation/increase in the size of lysosomes, induction of metallothionein, up-regulation of hypoxia inducible factor (hif-α), and metabolic changes associated with protein metabolism. Biological responses data were integrated with information about sediment chemical analysis and metal residue concentration in animal soft tissues. In conclusion, obtained results demonstrated the induction of CA in digestive gland of Mytilus galloprovincialis following to pollution exposure, and highlight its suitability to be integrated in a multimarker approach for the detection and characterization of the pollution induced-stress syndrome in this bioindicator organism.
Carbonic anhydrase integrated into a multimarker approach for the detection of the stress status induced by pollution exposure in Mytilus galloprovincialis: A field case study
Caricato R.Primo
Investigation
;Giordano M. E.Investigation
;Schettino T.;Mauceri A.;Ancora S.;Lionetto M. G.
Supervision
2019-01-01
Abstract
The aim of the present work was to study the sensitivity of the enzyme carbonic anhydrase (CA) to chemical pollution in Mytilus galloprovincialis digestive gland in the context of a multimarker approach in view of ecotoxicological biomonitoring and assessment application. The study was carried out by an active biomonitoring, based on the use of caged organisms from a single population, translocated to the areas of interest, the Augusta-Melilli-Priolo industrial site (eastern Sicily, Italy) and Brucoli (eastern Sicily, Italy) not affected by industrial contamination and selected as a reference site. Mussels exposed in field in Augusta showed a significant increase in the digestive gland CA activity and expression compared to the animals caged in the control site of Brucoli. The CA response in animals from the polluted site was paralleled by the increase of the fluorescence of LysoSensor green charged cells, indicative of proliferation/increase in the size of lysosomes, induction of metallothionein, up-regulation of hypoxia inducible factor (hif-α), and metabolic changes associated with protein metabolism. Biological responses data were integrated with information about sediment chemical analysis and metal residue concentration in animal soft tissues. In conclusion, obtained results demonstrated the induction of CA in digestive gland of Mytilus galloprovincialis following to pollution exposure, and highlight its suitability to be integrated in a multimarker approach for the detection and characterization of the pollution induced-stress syndrome in this bioindicator organism.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
