An immunocytochemical analysis according to the PAP-DAB procedure was performed on the chicken pontine and mesencephalic tegmentum, to study the distribution here of the neurons reacting to anti-cyclic-somatostatin-14 polyclonal rabbit antibodies. Immunoreactivity was not increased by any pretreatment; positive and negative controls of the immunoreaction were carried out. A positivity was chiefly detected: 1. in perikarya of the inferior half of the mesencephalic tegmentum, belonging probably to the n. mesencephalicus profundus cranially, and to the n. loci coerulei caudally; 2. in the perikarya of the cranial half of the mesencephalic tegmentum, belonging to the n. tegmentalis latero-dorsalis; 3. in perikarya of the cranial part of the n. interpeduncularis; 4. in long neuronal processes (a) of the pontine tegmentum – situated mainly in the dorsal half and running longitudinally -, (b) at the ponto-mesencephalic border – oriented almost transversally -, (c) of the mesencephalic tegmentum – placed mainly in its ventral half and running longitudinally; 5. in short neuronal processes within the periaqueductal grey.
Somatostatin-like immunoreactivity in the chicken brain stem. II. The pons and the tegmentum of the mesencephalon.
NICOLARDI, Giuseppe;
1985-01-01
Abstract
An immunocytochemical analysis according to the PAP-DAB procedure was performed on the chicken pontine and mesencephalic tegmentum, to study the distribution here of the neurons reacting to anti-cyclic-somatostatin-14 polyclonal rabbit antibodies. Immunoreactivity was not increased by any pretreatment; positive and negative controls of the immunoreaction were carried out. A positivity was chiefly detected: 1. in perikarya of the inferior half of the mesencephalic tegmentum, belonging probably to the n. mesencephalicus profundus cranially, and to the n. loci coerulei caudally; 2. in the perikarya of the cranial half of the mesencephalic tegmentum, belonging to the n. tegmentalis latero-dorsalis; 3. in perikarya of the cranial part of the n. interpeduncularis; 4. in long neuronal processes (a) of the pontine tegmentum – situated mainly in the dorsal half and running longitudinally -, (b) at the ponto-mesencephalic border – oriented almost transversally -, (c) of the mesencephalic tegmentum – placed mainly in its ventral half and running longitudinally; 5. in short neuronal processes within the periaqueductal grey.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
