A highly selectivemolecularly imprinted polymer (MIP) for 1-methyladenosine (1-MA), an urinarymod- ified nucleoside used as cancer marker, was prepared and its use as solid-phase extraction (SPE) sorbent material was demonstrated. TheMIP was prepared by a very simple procedure usingmethacrylic acid as functional monomer and a mixture acetonitrile/water (4/1, v/v) as porogen, overcoming in this way the problems usually related to the imprinting of biological polar compounds. The MIP was tested in batch experiments in order to evaluate its binding properties and then used as SPE sorbent for the selective clean-up and pre-concentration of 1-MA. The extraction protocol was successfully applied to the direct extractionof 1-MAfromspikedhumanurine indicating that theMIP allowed 1-MAto be pre-concentrated while simultaneously interfering compounds were removed from the matrix.
Molecularly imprinted polymers for solid-phase extraction of 1-methyladenosinefrom human urine
VASAPOLLO, Giuseppe;SCORRANO, SONIA;LONGO, Luigia
2010-01-01
Abstract
A highly selectivemolecularly imprinted polymer (MIP) for 1-methyladenosine (1-MA), an urinarymod- ified nucleoside used as cancer marker, was prepared and its use as solid-phase extraction (SPE) sorbent material was demonstrated. TheMIP was prepared by a very simple procedure usingmethacrylic acid as functional monomer and a mixture acetonitrile/water (4/1, v/v) as porogen, overcoming in this way the problems usually related to the imprinting of biological polar compounds. The MIP was tested in batch experiments in order to evaluate its binding properties and then used as SPE sorbent for the selective clean-up and pre-concentration of 1-MA. The extraction protocol was successfully applied to the direct extractionof 1-MAfromspikedhumanurine indicating that theMIP allowed 1-MAto be pre-concentrated while simultaneously interfering compounds were removed from the matrix.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
