Only few reports have shown protein expression of the Fc receptors (FcRs) molecules on human salivary gland cells. In this study we investigate a possible upregulation of FcRs following anti-Ro and anti-La autoantibodies treatment. Anti-Ro and anti-La autoantibodies were puriWed from IgG fractions obtained from 14 patients with primary Sjögren’s syndrome (SS), using Sepharose 4B-Ro and Sepharose 4B-La aYnity columns. Flow cytometry and RT-PCR were used to study the FCRI, FCRII and FCRIII receptors expression and upregulation by anti- Ro and anti-La on a salivary gland cell line. The present data document that the anti-Ro and anti-La autoantibodies determine an increase of the FcRs expression on salivary gland cells, and provide evidence that both the high aYnity FcRI and the low aYnity FcRII and FcRIII are overexpressed. Treatment with IgG isolated from healthy donors had no eVect on the basal FCRs expression.
Modulation of the Fcgamma receptors induced by anti-Ro and anti-La autoantibodies: observations in salivary gland cells
LOFRUMENTO, Dario Domenico;
2008-01-01
Abstract
Only few reports have shown protein expression of the Fc receptors (FcRs) molecules on human salivary gland cells. In this study we investigate a possible upregulation of FcRs following anti-Ro and anti-La autoantibodies treatment. Anti-Ro and anti-La autoantibodies were puriWed from IgG fractions obtained from 14 patients with primary Sjögren’s syndrome (SS), using Sepharose 4B-Ro and Sepharose 4B-La aYnity columns. Flow cytometry and RT-PCR were used to study the FCRI, FCRII and FCRIII receptors expression and upregulation by anti- Ro and anti-La on a salivary gland cell line. The present data document that the anti-Ro and anti-La autoantibodies determine an increase of the FcRs expression on salivary gland cells, and provide evidence that both the high aYnity FcRI and the low aYnity FcRII and FcRIII are overexpressed. Treatment with IgG isolated from healthy donors had no eVect on the basal FCRs expression.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
