In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy.

A novel Detection Strategy for Odorant Molecules Based on Controlled Bioengineering of Rat Olfactory Receptor I7

REGGIANI, Lino;PENNETTA, Cecilia;ALFINITO, ELEONORA;
2007-01-01

Abstract

In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11587/300589
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