Red berries of R. aculeatus, a wild shrub typical of Mediterranean Europe and Africa, were investigated for the 1st time to det. the profile of anthocyanins. The pigments were extd. from the skins of the berries with 0.1% HCl in methanol, purified using a C-18 solid phase cartridge, and identified by means of high-performance liq. chromatog. (HPLC)-diode array detection-mass spectrometry anal. Information from HPLC profiles, sapon., and acid hydrolysis of the anthocyanins showed that the major anthocyanins were pelargonidin 3-O-rutinoside (64%), pelargonidin 3-O-glucoside (16%), and pelargonidin 3-O-trans-p-coumarylglucoside (13%). The attractive color of R. aculeatus berries and the great abundance of the plant in the south of Italy make these berries a new and promising source of natural colorants.
Determination of Anthocyanins in Ruscus aculeatus L. berries
LONGO, Luigia;VASAPOLLO, Giuseppe
2005-01-01
Abstract
Red berries of R. aculeatus, a wild shrub typical of Mediterranean Europe and Africa, were investigated for the 1st time to det. the profile of anthocyanins. The pigments were extd. from the skins of the berries with 0.1% HCl in methanol, purified using a C-18 solid phase cartridge, and identified by means of high-performance liq. chromatog. (HPLC)-diode array detection-mass spectrometry anal. Information from HPLC profiles, sapon., and acid hydrolysis of the anthocyanins showed that the major anthocyanins were pelargonidin 3-O-rutinoside (64%), pelargonidin 3-O-glucoside (16%), and pelargonidin 3-O-trans-p-coumarylglucoside (13%). The attractive color of R. aculeatus berries and the great abundance of the plant in the south of Italy make these berries a new and promising source of natural colorants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
