The preparation and circular dichroic (CD) studies of self-complimentary 8-mer DNA sequences with a porphyrin at the 3- end are presented. Electronic interaction between the two porphyrins (the interchromophoric distance is in the range of 28-40 Å), attached to both ends of the double-stranded helix, gives rise to a long-range exciton-coupled CD in the visible region (400-450 nm). The porphyrin chromophores act as sensitive probes of geometrical changes in the DNA backbone and sensitively reflect the double-strand to single-strand transition. This study demonstrates the possibility of using exciton-coupled porphyrin CDs for conformational studies of DNA.
Titolo: | Synthesis and circular dichroism of tetraarylporphyrin-oligonucleotide conjugates |
Autori: | |
Data di pubblicazione: | 2005 |
Rivista: | |
Abstract: | The preparation and circular dichroic (CD) studies of self-complimentary 8-mer DNA sequences with a porphyrin at the 3- end are presented. Electronic interaction between the two porphyrins (the interchromophoric distance is in the range of 28-40 Å), attached to both ends of the double-stranded helix, gives rise to a long-range exciton-coupled CD in the visible region (400-450 nm). The porphyrin chromophores act as sensitive probes of geometrical changes in the DNA backbone and sensitively reflect the double-strand to single-strand transition. This study demonstrates the possibility of using exciton-coupled porphyrin CDs for conformational studies of DNA. |
Handle: | http://hdl.handle.net/11587/106001 |
Appare nelle tipologie: | Articolo pubblicato su Rivista |