Brush-border membrane (BBM) vesicles were prepared from the intestine of yellow eels (Anguilla anguilla) by selective precipitation of all other cellular components. The activity of a Na-substrate cotransporter was evaluated by measuring the rate of decay of an inside negative membrane potential with a voltage-sensitive fluorescent dye; the rate of fluorescence quenching decay in the presence of Na was subtracted from the rate of decay in the presence of both cosubstrates. The kinetics of Na-dependent amino acid transport systems were then determined for the neutral amino acids and their N-methylated analogues. It was concluded that N-methylglycine, N-methylalanine and α-(methylamino)-isobutyric acid are transported by the Na-dependent carrier responsible for proline translocation, which is distinct from the one responsible for glycine, alanine and α-aminoisobutyric acid translocations.

Do neutral amino acids and their N-methylated analogues share the same Na-dependent carrier in brush-border membrane from the eel intestine?

MAFFIA, Michele;VILELLA, Sebastiano;STORELLI, Carlo
1990

Abstract

Brush-border membrane (BBM) vesicles were prepared from the intestine of yellow eels (Anguilla anguilla) by selective precipitation of all other cellular components. The activity of a Na-substrate cotransporter was evaluated by measuring the rate of decay of an inside negative membrane potential with a voltage-sensitive fluorescent dye; the rate of fluorescence quenching decay in the presence of Na was subtracted from the rate of decay in the presence of both cosubstrates. The kinetics of Na-dependent amino acid transport systems were then determined for the neutral amino acids and their N-methylated analogues. It was concluded that N-methylglycine, N-methylalanine and α-(methylamino)-isobutyric acid are transported by the Na-dependent carrier responsible for proline translocation, which is distinct from the one responsible for glycine, alanine and α-aminoisobutyric acid translocations.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11587/105211
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 3
social impact