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EnglishBrush-border membrane (BBM) vesicles were prepared from the intestine of yellow eels (Anguilla anguilla) by selective precipitation of all other cellular components. The activity of a Na-substrate cotransporter was evaluated by measuring the rate of decay of an inside negative membrane potential with a voltage-sensitive fluorescent dye; the rate of fluorescence quenching decay in the presence of Na was subtracted from the rate of decay in the presence of both cosubstrates. The kinetics of Na-dependent amino acid transport systems were then determined for the neutral amino acids and their N-methylated analogues. It was concluded that N-methylglycine, N-methylalanine and α-(methylamino)-isobutyric acid are transported by the Na-dependent carrier responsible for proline translocation, which is distinct from the one responsible for glycine, alanine and α-aminoisobutyric acid translocations.
| Titolo: | Do neutral amino acids and their N-methylated analogues share the same Na-dependent carrier in brush-border membrane from the eel intestine? | |
| Autori: | ||
| Data di pubblicazione: | 1990 | |
| Rivista: | ||
| Abstract: | Brush-border membrane (BBM) vesicles were prepared from the intestine of yellow eels (Anguilla anguilla) by selective precipitation of all other cellular components. The activity of a Na-substrate cotransporter was evaluated by measuring the rate of decay of an inside negative membrane potential with a voltage-sensitive fluorescent dye; the rate of fluorescence quenching decay in the presence of Na was subtracted from the rate of decay in the presence of both cosubstrates. The kinetics of Na-dependent amino acid transport systems were then determined for the neutral amino acids and their N-methylated analogues. It was concluded that N-methylglycine, N-methylalanine and α-(methylamino)-isobutyric acid are transported by the Na-dependent carrier responsible for proline translocation, which is distinct from the one responsible for glycine, alanine and α-aminoisobutyric acid translocations. | |
| Handle: | http://hdl.handle.net/11587/105211 | |
| Appare nelle tipologie: | Articolo pubblicato su Rivista |
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