Using as a template genomic DNA extracted from the cultivar Ofanto of durum wheat (Triticum turgidum var. durum Desf.), and as DNA primers two oligonucleotides spanning Lipoxygenase-1 (LOX-1) gene region, evolutionary conserved in different plants, we have amplified in a PCR reaction a 1200 bp fragment. This DNA fragment has been subcloned and used to prepare probes in Slot-blot experiments. Our aim was to detect the expression LOX gene(s), at level of RNA, in different cultivars of Durum wheat. Preliminary results indicates that there are significant variations in the expression of LOX gene(s) in the tested samples, and a good negative correlation of LOX-1 mRNA levels with both semolina beta-carotene content and semolina yellow index of durm wheat varieties utilized. The physiological significance of this relation will be discussed.

Differential expression of Lipoxygenase genes among Durhum wheat cultivars.

ALIFANO, Pietro;
1998-01-01

Abstract

Using as a template genomic DNA extracted from the cultivar Ofanto of durum wheat (Triticum turgidum var. durum Desf.), and as DNA primers two oligonucleotides spanning Lipoxygenase-1 (LOX-1) gene region, evolutionary conserved in different plants, we have amplified in a PCR reaction a 1200 bp fragment. This DNA fragment has been subcloned and used to prepare probes in Slot-blot experiments. Our aim was to detect the expression LOX gene(s), at level of RNA, in different cultivars of Durum wheat. Preliminary results indicates that there are significant variations in the expression of LOX gene(s) in the tested samples, and a good negative correlation of LOX-1 mRNA levels with both semolina beta-carotene content and semolina yellow index of durm wheat varieties utilized. The physiological significance of this relation will be discussed.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11587/102981
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