Characterization of the lipid fraction of raw cow and buffalo milk samples, collected in different breeding areas in Apulia, a region of southern Italy, were performed by means of 1H NMR. The aim of this work was to establish whether FA compn. data obtained by 1H NMR can be used in the differentiation of buffalo and cow milk samples according to species. A complete assignment of the signals present in the spectrum was attempted by COSY, hetero-nuclear multiple-quantum coherence, and heteronuclear single-quantum coherence spectra. Quantification of FA was carried out by inserting the integrals of particular peaks in suitable calcns. Multivariate statistical anal., conducted on the results of the quantification, permitted buffalo and cow milks to be distinguished.
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