A proteomics-based approach was exploited to individuate peptide sequences having the immunogenic potential to evoke humoral response. The epitope search utilized two parameters: the similarity level of the peptide sequence to the host's proteins, and the peptide capability to bind to the major histocompatibility complex class II mols. By this approach, the human papillomavirus 16 E749-63 RAHYNIVTFCCKCDS peptide was individuated as the immunogenic epitope recognized by an anti-HPV16 E7 monoclonal antibody raised against the full-length viral oncoprotein. In this report, two-dimensional NMR spectroscopic expts. unequivocally probe the HPV16 E7 epitope individuation.
NMR probing of in silico identification of anti-HPV16 E7 mAb linear peptide epitope.
FANIZZI, Francesco Paolo;LUCCHESE GUGLIELMO;
2004-01-01
Abstract
A proteomics-based approach was exploited to individuate peptide sequences having the immunogenic potential to evoke humoral response. The epitope search utilized two parameters: the similarity level of the peptide sequence to the host's proteins, and the peptide capability to bind to the major histocompatibility complex class II mols. By this approach, the human papillomavirus 16 E749-63 RAHYNIVTFCCKCDS peptide was individuated as the immunogenic epitope recognized by an anti-HPV16 E7 monoclonal antibody raised against the full-length viral oncoprotein. In this report, two-dimensional NMR spectroscopic expts. unequivocally probe the HPV16 E7 epitope individuation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
