In this study, we compared two widely used methodologies for the decellularization of plant tissues intended for recellularization with mammalian cells. The scaffold’s performance was evaluated in terms of porosity, elastic modulus, and cell viability. The influence of decellularization protocols and subsequent post-treatment processes on cell survival was assessed in accordance with ISO 10993-5:2009 and ISO 10993-12:2009 standards, using the Saos-2 cell line. Our results indicate that the hypochlorite-based decellularization protocol successfully decellularized the samples within 5 hours, yielding an elastic modulus of 104.5 ± 32. 2 kPa and a cell survival rate above 70% after 24 and 72 hours. The SDS-based decellularization protocol produced an elastic modulus of 117.5 ± 31.8 kPa and achieved cell survival rates above 70% after 24 and 72 hours, following washing steps with Tris-HCl buffer at either pH 6.6 or pH 8.8.
Impact of the decellularization procedure of plant-based scaffolds on cellular viability
Vargas-Ovalle M. I.
;Madaghiele M.;Di Chiara Stanca B.;Siculella L.;Demitri C.
2025-01-01
Abstract
In this study, we compared two widely used methodologies for the decellularization of plant tissues intended for recellularization with mammalian cells. The scaffold’s performance was evaluated in terms of porosity, elastic modulus, and cell viability. The influence of decellularization protocols and subsequent post-treatment processes on cell survival was assessed in accordance with ISO 10993-5:2009 and ISO 10993-12:2009 standards, using the Saos-2 cell line. Our results indicate that the hypochlorite-based decellularization protocol successfully decellularized the samples within 5 hours, yielding an elastic modulus of 104.5 ± 32. 2 kPa and a cell survival rate above 70% after 24 and 72 hours. The SDS-based decellularization protocol produced an elastic modulus of 117.5 ± 31.8 kPa and achieved cell survival rates above 70% after 24 and 72 hours, following washing steps with Tris-HCl buffer at either pH 6.6 or pH 8.8.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
