Arsenic uptake by plants occurs primarily through the root but it is not well known. Dittrichia viscosa uptake and translocation of As is not fully understood and some data are contradictory. In one study is reported that As is fully translocated to D. viscosa shoots and volatilized, while in another is show that As is stabilized in the roots. In a previous work we studied the accumulation of As[III] and As[V], confirming the high variability among individuals. We established several experimental clonal populations to perform a functional study on the aquaporin NIP1.1. D. viscosa is not a hyperaccumulator plant but can grow in high drought conditions while still producing large biomass, even tolerating significant concentrations of As[III], As[V] and Cd[II]. In spite of these remarkable characteristics, adaptive modification of performances is not predictable in wild populations. Genetic stability is a desirable trait and interest in the genetic improvement of this plant species is increasing. We test a strategy that take in consideration the use of a clonal population of D. viscosa with a defined NIP1.1 expression level and the in-vitro micropropagation technique to quickly scaleup the number of plants to perform specific HMs phytoremediation of contaminated areas.
Dittrichia viscosa selection strategy based on Nip1.1 Gene for Arsenic phytoremediation
Barozzi F.;Anglana C.;Capaci P.;Di Sansebastiano G. P.
2023-01-01
Abstract
Arsenic uptake by plants occurs primarily through the root but it is not well known. Dittrichia viscosa uptake and translocation of As is not fully understood and some data are contradictory. In one study is reported that As is fully translocated to D. viscosa shoots and volatilized, while in another is show that As is stabilized in the roots. In a previous work we studied the accumulation of As[III] and As[V], confirming the high variability among individuals. We established several experimental clonal populations to perform a functional study on the aquaporin NIP1.1. D. viscosa is not a hyperaccumulator plant but can grow in high drought conditions while still producing large biomass, even tolerating significant concentrations of As[III], As[V] and Cd[II]. In spite of these remarkable characteristics, adaptive modification of performances is not predictable in wild populations. Genetic stability is a desirable trait and interest in the genetic improvement of this plant species is increasing. We test a strategy that take in consideration the use of a clonal population of D. viscosa with a defined NIP1.1 expression level and the in-vitro micropropagation technique to quickly scaleup the number of plants to perform specific HMs phytoremediation of contaminated areas.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
