The use of mosses in biotechnology is an ever-expanding area of interest. Traditionally, mosses have often been used as medicinal plants and nowadays can be a great source of secondary metabolites with pharmacological activity (Mishra et al., 2014). In agriculture formulations based on bryophyte extracts have been developed and used as natural pesticides, biopesticides or for their allelopathic effects (Nozaki et al., 2007). Another important aspect is their application as bioindicators for their ability to accumulate polluting substances, such as heavy metals, modifying, as response, their secondary metabolism. We showed as aquatic mosses can be used in phytoremediation/phytofiltration of polluted waters as a biofilter having the ability to absorb heavy metals such as Pb, Cd, and Cr in a short time (Papadia et al., 2020) and their ability to capture inorganic nanoparticles revealing a potential tool capable of purifying water from nanostructured materials reducing the toxicity associated with the ingestion of contaminated drinking water (De Matteis et al., 2021). To further expand research in the field of biotechnology, solid in vitro propagation culture protocols are needed and an extensive knowledge of in vitro adapted lines. Laboratory protocols for axenic in vitro culture of moss were adapted to three species of aquatic moss: Leptodictyum riparium (Amblystegiaceae), Vesicularia montagnei and Taxiphyllum barbieri (Hypnaceae). The anatomic characters for their description and physiologic monitoring were described and their biotechnological potential started to be explored. Treatments with phytohormones, cytokinin (BAP) and auxin (NAA), showed a different response in the three species. Selected the best growing condition, stereoscope was used to analyze different qualitative characters, and light microscope was used to observe and measure the laminal and alar cells of the leaf with differences between the three species after that cycle induction conditions were tested to study the various life stages. Not all species adapt completely growing in vitro and may be suitable for different purposes. The metabolic profile was also analyzed with 1H-NMR and analysis of metals and trace elements were analysed using ICP/AES to highlight the differences between the three species.
In vitro propagation of three species of aquatic mosses (Bryophyta), anatomic description and biotechnological potential.
Anglana C.;Capaci P.;Barozzi F.;Di Sansebastiano G. P.
2023-01-01
Abstract
The use of mosses in biotechnology is an ever-expanding area of interest. Traditionally, mosses have often been used as medicinal plants and nowadays can be a great source of secondary metabolites with pharmacological activity (Mishra et al., 2014). In agriculture formulations based on bryophyte extracts have been developed and used as natural pesticides, biopesticides or for their allelopathic effects (Nozaki et al., 2007). Another important aspect is their application as bioindicators for their ability to accumulate polluting substances, such as heavy metals, modifying, as response, their secondary metabolism. We showed as aquatic mosses can be used in phytoremediation/phytofiltration of polluted waters as a biofilter having the ability to absorb heavy metals such as Pb, Cd, and Cr in a short time (Papadia et al., 2020) and their ability to capture inorganic nanoparticles revealing a potential tool capable of purifying water from nanostructured materials reducing the toxicity associated with the ingestion of contaminated drinking water (De Matteis et al., 2021). To further expand research in the field of biotechnology, solid in vitro propagation culture protocols are needed and an extensive knowledge of in vitro adapted lines. Laboratory protocols for axenic in vitro culture of moss were adapted to three species of aquatic moss: Leptodictyum riparium (Amblystegiaceae), Vesicularia montagnei and Taxiphyllum barbieri (Hypnaceae). The anatomic characters for their description and physiologic monitoring were described and their biotechnological potential started to be explored. Treatments with phytohormones, cytokinin (BAP) and auxin (NAA), showed a different response in the three species. Selected the best growing condition, stereoscope was used to analyze different qualitative characters, and light microscope was used to observe and measure the laminal and alar cells of the leaf with differences between the three species after that cycle induction conditions were tested to study the various life stages. Not all species adapt completely growing in vitro and may be suitable for different purposes. The metabolic profile was also analyzed with 1H-NMR and analysis of metals and trace elements were analysed using ICP/AES to highlight the differences between the three species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
