Antitumor and Antiangiogenic Activities of [Pt(O,O' acac)(γ-acac)(DMS)] in human renal cell carcinoma

Tumor growth, tumor cell proliferation and microvessel density, in a xenograft model of RCC, developed by injection of Caki-1 cells in BALB/c nude mice, were investigated. Exposure of the Caki-1 cells to cisplatin and to [Pt(O,O’-acac)(γ-acac)(DMS)] resulted in a dose-dependent inhibition of cell survival. [Pt(O,O′-acac)(γ-acac)(DMS)] was much more effective than cisplatin in the inhibition of tumor growth, proliferation and angiogenesis in vivo as well as migration, tube formation and MMP-1, MMP-2 and MMP-9 secretion of endothelial cells, in vitro. Wherease, cisplatin exerted a higher cytotoxic activity on Huvecs, but did not affect tube formation and migration of endothelial cells. In addition, treatment of xenograft mice with [Pt(O,O′-acac)(γ-acac)(DMS)] decreased, VEGF, MMP-1 and MMP-2 expression in tumors.The anti-angiogenic and anti-tumor activities of [Pt(O,O’-acac)(γ-acac)(DMS)] providing a solid starting point for its validation as a suitable candidate for further pharmacological testing.