In this chapter, we discuss several immunohistochemical methods to monitor apoptosis and autophagy in two models, the cultured rat neuroblastoma cells and the developing rat cerebellum . Apoptosis and autophagy have been implicated in many physiological and pathological processes. Apoptotic cell death is characterized by membrane blebbing and nuclear chromatin condensation /fragmentation , alteration of cytoskeleton components and cytoplasmic organelles , and formation of membrane-enveloped apoptotic bodies that are rapidly phagocytosed by macrophages . Autophagy is a sequential set of events including double membrane formation and elongation, vesicle maturation , and finally delivery of the targeted materials to the lysosome . Platinum compounds can induce different apoptotic pathways in neural cell cultures and alter the normal balance between the formation and degradation of cellular proteins . These compounds also affect cell death by apoptosis and autophagy in the proliferating and differentiating cells of rat cerebellum in vivo .
In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis and Autophagy
FANIZZI, Francesco Paolo;
2015-01-01
Abstract
In this chapter, we discuss several immunohistochemical methods to monitor apoptosis and autophagy in two models, the cultured rat neuroblastoma cells and the developing rat cerebellum . Apoptosis and autophagy have been implicated in many physiological and pathological processes. Apoptotic cell death is characterized by membrane blebbing and nuclear chromatin condensation /fragmentation , alteration of cytoskeleton components and cytoplasmic organelles , and formation of membrane-enveloped apoptotic bodies that are rapidly phagocytosed by macrophages . Autophagy is a sequential set of events including double membrane formation and elongation, vesicle maturation , and finally delivery of the targeted materials to the lysosome . Platinum compounds can induce different apoptotic pathways in neural cell cultures and alter the normal balance between the formation and degradation of cellular proteins . These compounds also affect cell death by apoptosis and autophagy in the proliferating and differentiating cells of rat cerebellum in vivo .I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
